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  • How-to guides

    NAD+: Metabolic / Longevity research guide

    In short: NAD+ (nicotinamide adenine dinucleotide) is the central redox coenzyme of cellular metabolism and the obligatory substrate of the sirtuin, PARP and CD38 enzyme families — the molecule at the heart of mitochondrial-function and cellular-ageing research. It is studied in preclinical (cell and animal) research and is supplied strictly for laboratory use, not for human or clinical use.

    Not medical advice. NAD+ is a research compound. This guide does not provide dosing, diagnosis, therapy recommendations, or claims about effects in humans.

    NAD+ at a glance

    Compound type
    Research peptide
    Research category
    Metabolic / Longevity
    Also known as
    NAD+, nicotinamide adenine dinucleotide, β-NAD, β-nicotinamide adenine dinucleotide, diphosphopyridine nucleotide (DPN), coenzyme I
    Molecular weight
    663.43 Da
    Research-grade purity
    >99% HPLC
    Evidence context
    Preclinical / research use only
    Regulatory status
    Not an approved medicine. Supplied as a research compound
    Last reviewed
    5 August 2026

    What to know first

  • The central redox coenzyme of metabolism — cycles between NAD+ and NADH to carry electrons through glycolysis, the TCA cycle and β-oxidation
  • Delivers reducing equivalents to complex I of the electron-transport chain, the entry point of mitochondrial ATP production
  • Obligatory substrate for the sirtuin deacylases (SIRT1–SIRT7) studied in metabolic and cellular-ageing models
  • Required co-substrate for PARP1/PARP2 during DNA-damage detection and repair signalling
  • Details and limits sit in the sections below. Research use only.

    What NAD+ is

    NAD+ is the cell’s central redox coenzyme and the fuel for the sirtuin, PARP and CD38 signalling enzymes — the single molecule that links energy metabolism to DNA repair, gene regulation and cellular ageing.

    One-paragraph overview from our research datasheet. Still scientific, but faster to read than the full mechanism list below.

    NAD+ (nicotinamide adenine dinucleotide) is the central redox coenzyme of cellular metabolism and the obligatory substrate of the sirtuin, PARP and CD38 enzyme families — the molecule at the heart of mitochondrial-function and cellular-ageing research.

    Research contexts

    Peer-reviewed papers discuss NAD+ in specific lab settings. The points below reflect research questions, not health claims.

    Research vs. personal use: Literature describes experiments in cells and animals. That is distinct from real-world use. Our products are for laboratory research only.

    Typical study contexts

  • Mitochondrial stress, NAD+/redox biology and nutrient-sensing pathways.
  • Enzyme activity, gene expression or endurance-style animal readouts.
  • The central redox coenzyme of metabolism — cycles between NAD+ and NADH to carry electrons through glycolysis, the TCA cycle and β-oxidation.
  • Delivers reducing equivalents to complex I of the electron-transport chain, the entry point of mitochondrial ATP production.
  • Obligatory substrate for the sirtuin deacylases (SIRT1–SIRT7) studied in metabolic and cellular-ageing models.
  • Required co-substrate for PARP1/PARP2 during DNA-damage detection and repair signalling.
  • Why Metabolic / Longevity research matters

    Researchers study these compounds for mitochondrial function and cellular energy stress. Ageing and metabolic disease models are common.

    Mechanisms (technical review)

    Our datasheet lists mechanistic themes from preclinical work. These are research endpoints, not health claims.

  • The central redox coenzyme of metabolism — cycles between NAD+ and NADH to carry electrons through glycolysis, the TCA cycle and β-oxidation
  • Delivers reducing equivalents to complex I of the electron-transport chain, the entry point of mitochondrial ATP production
  • Obligatory substrate for the sirtuin deacylases (SIRT1–SIRT7) studied in metabolic and cellular-ageing models
  • Required co-substrate for PARP1/PARP2 during DNA-damage detection and repair signalling
  • Cleaved by CD38 / CD157 to generate cyclic ADP-ribose, a calcium-mobilising second messenger
  • Resynthesised through the NAMPT salvage pathway — the rate-limiting step most NAD+ research targets
  • Lab handling & preparation

  • Storage: Lyophilised powder: store in freezer (−20 °C), protected from light and moisture. Reconstituted: refrigerate 1–6 °C and use promptly — NAD+ in solution is hydrolytically unstable and degrades faster at alkaline pH and at room temperature. Use within the validated stability window for the specific batch.. See the storage guide.
  • Research dosing context: Literature typically discusses 100–1000 mg per preparation in published research protocols (route-dependent). Intermittent dosing in published research protocols; intravenous infusion and subcutaneous routes are the most commonly described Published intravenous NAD+ research protocols span roughly 250–1000 mg per infusion delivered slowly over several hours, because rapid administration is poorly tolerated in the reported literature; smaller subcutaneous amounts appear in exploratory work. Oral NAD+ itself is largely hydrolysed in the gut, which is why precursor strategies (nicotinamide riboside, nicotinamide mononucleotide) dominate the oral literature while injectable protocols use intact NAD+. These figures describe the research context and are not a dosing recommendation. Material is for laboratory research only.
  • Preparation: Follow the reconstitution guide. Confirm volumes with the calculator.
  • Purity check: Use the COA reading guide and the published NAD+ Certificate of Analysis.
  • Common Questions People Are Asking

    What is NAD+ used for in research?

    NAD+ is studied as the central redox coenzyme of metabolism and as the consumed substrate of the sirtuin, PARP and CD38 enzyme families. Research examines mitochondrial electron transport and ATP production, sirtuin-mediated gene regulation, PARP-dependent DNA-repair signalling, calcium second-messenger generation, circadian oscillation of the NAD+ pool, and the age-related decline of tissue NAD+ in cellular-senescence models. It is supplied here for laboratory research only and is not for human use.

    What is the difference between NAD+ and NADH?

    They are the two halves of one redox couple. NAD+ is the oxidised form; it accepts a hydride ion (two electrons and a proton) to become NADH, the reduced form, which then donates that hydride elsewhere — most importantly to complex I of the mitochondrial electron-transport chain — regenerating NAD+. Cells hold the free cytosolic pool strongly toward NAD+, and that ratio is what makes the oxidative steps of glycolysis thermodynamically favourable.

    How does NAD+ differ from NMN and nicotinamide riboside?

    NMN and NR are precursors — smaller molecules that cells convert into NAD+ through the salvage pathway. NAD+ is the finished coenzyme. The practical difference is delivery: NAD+ is large and doubly charged, so it crosses membranes poorly and is broken down extracellularly by CD38 and CD73, which is why most oral human research uses NR or NMN while intact NAD+ research uses parenteral routes.

    Why does NAD+ decline with age?

    Model-organism data point to both sides of the ledger changing at once. Supply falls as NAMPT salvage-pathway activity decreases, while demand rises: PARP enzymes consume more NAD+ as DNA damage accumulates, and CD38 expression increases with inflammatory ageing. Since sirtuins draw on the same pool, the widely studied hypothesis is that sirtuin activity falls in ageing tissue because its substrate does.

    How should NAD+ be stored?

    Keep the lyophilised powder frozen at −20 °C, protected from light and moisture. After reconstitution in bacteriostatic water or sterile saline, refrigerate at 1–6 °C and use promptly — NAD+ hydrolyses in solution, and degradation accelerates at alkaline pH and at room temperature. Aliquot after reconstitution and avoid repeated freeze-thaw cycles where the protocol allows.

    Is this page medical advice? Can I use NAD+ for my health?

    No, and no. This article is educational only. We do not provide dosing, medical recommendations, or health claims. Our products are sold strictly for laboratory research, not for personal use of any kind.

    Where do I find NAD+ specs, purity certificates and pricing?

    Open the shop listing via “View product details.” There you will see batch specs, the Certificate of Analysis (COA), concentration, purity grade, and available SKUs with current pricing.

    Related peptide guides

    Other compounds researchers often read about alongside NAD+.

  • SS-31 - companion guide
  • MOTS-C - companion guide
  • Glutathione - companion guide
  • Epitalon - companion guide
  • Scientific sources & further reading

    Primary literature and registries for NAD+, plus mainstream coverage of the peptide category. Research use only - not medical advice.

    Databases & literature:

  • PubMed: peer-reviewed literature on NAD+
  • ClinicalTrials.gov: registered studies on NAD+
  • NAD+: Wikipedia
  • Peptides in the news:

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  • Ready to order? View full product specs

    Access concentration, batch info, variants, and current pricing on our shop.

    Also known as: NAD+, nicotinamide adenine dinucleotide, β-NAD, β-nicotinamide adenine dinucleotide, diphosphopyridine nucleotide (DPN), coenzyme I

    PRECISION. PURITY. PERFORMANCE.

    Research peptides at >99% HPLC-verified purity, third-party tested by Janoshik Analytical & Freedom Diagnostics, with Certificates of Analysis published per released batch. Supplied strictly for laboratory research use.

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